Table 1 Experiments completed for each study (i.e. set of animals)

Study #1

Thermal and mechanical hypersensitivity assay (Figure 3).

Neurite retraction study using PGP 9.5 (n = 3/group randomly selected, Figure 2).

Mewo (control)

Poka (VZV)

n = 10

n = 10

Study #2

Place escape/avoidance paradigm (Figure 4).

VZV infection detected with IE62 marker (Figure 1).

PBS (vehicle)

Mewo (control)

Poka (VZV virus)

n = 6

n = 6

n = 6

Study #3

Gabapentin assay (Figure 5).

Mewo/vehicle

Mewo/gabapentin

Poka/vehicle

Poka/gabapentin

n = 5

n = 5

n = 5

n = 5

Study #4

Sex differences study comparing males and females (Figure 6).

Mewo/female

Mewo/male

Poka/female

Poka/male

n = 24

n = 7–15

n = 25

n = 7–15

Study #5

OVX females as compared to males (Sprague Dawley and Long Evans, Figure 7).

VZV infection detected with IE62 marker (Sprague-Dawley rats, no figure).

Mewo/male

Mewo/female

Mewo/OVX female

Poka/male

Poka/female

Poka/OVX female

n = 5

n = 4

n = 4

n = 7

n = 6

n = 6

n = 5 (Long Evans) for all treatment groups

Study #6

Female estrous cycle study (Figure 8).

Mewo diestrus

Mewo proestrus/estrus

Poka diestrus

Poka proestrus/estrus

n ≥ 8

n ≥ 8

n ≥ 8

n ≥ 8