Table 1 Loci of selected DRD and GRIN2B SNPs and the corresponding primer pairs used for high resolution melt analysis
SNP | Chromosome | Location | Allele changes | Amino acid changes | Forward primer (5′➔3′) | Reverse primer (5′➔3′) | Amplicon size (bp) |
|---|---|---|---|---|---|---|---|
DRD1 rs4532 | chr 5 (q35.2) | 19681423 | C > T | Nil | GAACAGAGAAGTCCCTCTCCAC | CTGGAAATCTGACTGACCCCTA | 147 |
DRD1 rs4867798 | chr 5 (q35.2) | 19679572 | T > C | Nil | GGGCTCTTCTTAAGTTGGCTTT | GGACACAGATAAATGCAAGGTG | 189 |
DRD1 rs265981 | chr 5 (q35.2) | 174870940 | T > C | Nil | GCTCTCTCCCAAGGAAGCTC | GTGCGTTTGGGGAAAGGATC | 141 |
DRD2/ANKK1 rs1800497 | chr 11 (q23.2) | 16833244 | C > T | Glu > Lys | CTCTAGGAAGGACATGATGCCC | GCAACACAGCCATCCTCAAAG | 128 |
DRD2 rs104894220 | chr 11 (q23.2) | 16850073 | G > A | Val > Ile | CATGCCCATGCTGTACAATACG | GTACCTGCGTTATTGAGTCCGA | 126 |
DRD2 rs144999500 | chr 11 (q23.2) | 16845792 | G > A | Pro > Leu | GAGCATCTGAGTGGCTTTCTTCTC | GAGAAGAATGGGCATGCCAAAG | 150 |
DRD3 rs3732783 | chr 3 (q13.31) | 20385935 | T > C | Ala > Ala | AGTAGGAGAGGGCATAGTAGGC | CTGGGCTATGGCATCTCTGAG | 116 |
DRD3 rs6280 | chr 3 (q13.31) | 20385961 | C > T | Gly > Ser | AGTAGGAGAGGGCATAGTAGGC | CTGGGCTATGGCATCTCTGAG | 116 |
DRD4 rs1800443 | chr 11 (p15.5) | 579830 | T > G | Val > Gly | TACTGTGCGGCCTCAACGAC | GGGTAGGAAGAAGGAGCACAC | 104 |
DRD5 rs144132215 | chr 4 (p16.1) | 966018 | G > T | Gly > Trp | GTCCATCCTCATCTCCTTCATTCC | CTGGAGTCACAGTTCTCTGCAT | 159 |
GRIN2B rs7301328 | chr 12 (p13.1) | 6778901 | G > C | Pro > Pro | CTCCCTGCAGCCCCTTTTTA | CGCCCAGATCCTCGATTTCA | 109 |