Figure 2
From: Aquaporin-4 expression in distal myopathy with rimmed vacuoles
Pathology of muscle. Hematoxylin and eosin staining (A). Modified Gomori-trichrome staining (B) and immunohistochemical examination of aquaporin-4 (AQP4) (inset) in the serial section (Note the arrow-heads). Immunohistochemical examination of AQP4 (C). Immunofluorescence analysis of AQP4 shows that the rimmed vacuoles (RVs) and the areas surrounding the RVs are often strongly AQP4 positive (D, arrow-heads) but not always so (D, arrow). Negative controls, without incubations with anti-AQP4 antibody (E). AQP4 expression in normal control muscle (F). Double immunofluorescence analysis of AQP4 and myosin heavy chain-fast (MHCF) in the normal control muscle (G1-G3).