Figure 7
Immunoreactivity of fission/fusion proteins in brain mitochondria isolated from mouse brain following NMN treatment. (A) Representative Western blot images of fission (DRP1 and phosphorylated form P616-DRP1) and fusion (OPA1 and MFN2) proteins. (B-F) Graphs showing quantification of the Western blots in (A). The OPA1 oligomeric complex comprises the long (L) fusogenic form and the short (S) form [32]. Voltage-dependent anion channel (VDAC) was utilized as a loading control. Data are presented as the average DRP, P616-DRP1, OPA1L OPA1S, MFN2 ± SE. N = 5–7 separate animals per group. **p < 0.05.