Figure 5

Mthfd2 expression level in ribozyme clones and in FRDA patient's fibroblasts. (A) Quantitative-RT-PCR analyses were performed on three different cellular RNA extracts of R2^m and R2C1 clones, comparing the expression of murine Mthfd2 transcript to the reference Hprt transcript. The R2C1 cells used for these experiments present the "adjusted" frataxin residual level of 29 ± 7%. (B). Quantitative-RT-PCR analyses were performed on two different cellular RNA extracts of FRDA (n = 3) and control (n = 5) fibroblasts, comparing expression of the human MTHFD2 transcript to 18S ribosome transcript. ***p ≤ 0.005.