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Table 2 Primers for PCR amplification of full-length human aquaporin 4 (hAQP4)

From: An immunoassay that distinguishes real neuromyelitis optica signals from a labeling detected in patients receiving natalizumab

Gene

Primer sequences

hAQP4-M23

Forward : 5′-ACTCCTCGAGGGCGGTGGGGTAAGTGTGGAC -3′

 

Reverse: 5′-ACTCCCCGGGAATGGGTGGAAGGAAATCTGA -3′

  1. Primers were designed using the Primer Express software (Applied-Biosystems). Sequences of restriction enzymes used for cloning purpose are indicated: CTCGAG: Xho1 and CCCGGG: Xma1.
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BMC Neurology

ISSN: 1471-2377

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