High sensitivity of an ELISA kit for detection of the gamma-isoform of 14-3-3 proteins: usefulness in laboratory diagnosis of human prion disease

Table 3 Summary of the detection of ELISA of 14-3-3 protein, the detection of WB method of 14-3-3 protein and in CSF for 21 patients with early-stage CJD

Age	Sex	CJD type		d.w.	CSF				MRI
					ELISA of 14-3-3 protein AU/ml	the detection of WB method of 14-3-3a protein	the detection of WB method of 14-3-3γ protein	Real-time QUIC	DWI
71	m	sp	probable	0	4, 157	-	-	+	+
77	m	sp	probable	2	10, 812	+	+	+	+
64	f	sp	probable	4	11, 812	+	+	+	+
73	m	sp	probable	4	3, 850	+	+	-	+
67	m	sp	probable	4	10, 814	+	+	+	+
76	m	sp	probable	4	6, 772	+	+	+	+
80	f	sp	probable	4	9, 850	+	+	+	+
63	f	sp	probable	4	2, 987	-	-	-	+
67	m	sp	probable	4	3, 553	+	+	+	+
70	f	sp	probable	4	3, 729	-	-	-	+
63	m	sp	probable	4	6, 133	+	+	+	+
63	m	sp	probable	4	8, 297	+	+	+	+
67	f	sp	probable	5	3, 372	+	-	-	-
74	m	sp	probable	5	7, 184	+	+	+	+
69	f	sp	definite	6	5, 600	+	+	+	+
54	f	sp	definite	6	1, 897	-	-	-	+
70	f	ia	probable	6	9, 460	+	+	+	-
70	f	fa	probable	6	4, 417	+	+	+	+
64	f	sp	probable	6	3, 888	+	+	+	+
51	f	ia	probable	6	6, 313	+	+	+	+
74	f	sp	probable	6	6, 133	+	+	+	+

sp = Sporadic CJD; ia = iatrogenic CJD; fa = familiar CJD; d.l. = diagnostic level based on the WHO and the Masters criteria; d.w. = duration from the onset of the disease to the diagnostic examination. In all 21 cases, codon 129 of PRNP was Met/Met homozygous, whereas codon 219 was Glu/Glu homozygous. Total protein contents of all the patients stayed within the normal range. All patients in this study were Asian.

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